Dominant alleles identify SET domain residues required for histone methyltransferase of Polycomb repressive complex 2
P Joshi, EA Carrington, L Wang, CS Ketel… - Journal of biological …, 2008 - ASBMB
Polycomb gene silencing requires histone methyltransferase activity of Polycomb repressive
complex 2 (PRC2), which methylates lysine 27 of histone H3. Information on how PRC2
works is limited by lack of structural data on the catalytic subunit, Enhancer of zeste (E (Z)),
and the paucity of E (z) mutant alleles that alter its SET domain. Here we analyze missense
alleles of Drosophila E (z), selected for molecular study because of their dominant genetic
effects. Four missense alleles identify key E (Z) SET domain residues, and a fifth is located in …
complex 2 (PRC2), which methylates lysine 27 of histone H3. Information on how PRC2
works is limited by lack of structural data on the catalytic subunit, Enhancer of zeste (E (Z)),
and the paucity of E (z) mutant alleles that alter its SET domain. Here we analyze missense
alleles of Drosophila E (z), selected for molecular study because of their dominant genetic
effects. Four missense alleles identify key E (Z) SET domain residues, and a fifth is located in …
