Spermine-NBD as fluorescent probe for studies of the polyamine transport system in Leishmania donovani
E Jagu, S Pomel, S Pethe, JC Cintrat… - Bioorganic & Medicinal …, 2019 - Elsevier
Bioorganic & Medicinal Chemistry Letters, 2019•Elsevier
This study describes the synthesis of fluorescent probes as potential substrates for the
polyamine transport system (PTS) of Leishmania donovani. A competitive radioassay was
used to determine the most efficient probe. We observed that the conjugate spermine-
nitrobenzofurazan (Spm-NBD) was able to compete with [3 H]-spermidine in L. donovani at
a potent IC 50 of 60 µM.
polyamine transport system (PTS) of Leishmania donovani. A competitive radioassay was
used to determine the most efficient probe. We observed that the conjugate spermine-
nitrobenzofurazan (Spm-NBD) was able to compete with [3 H]-spermidine in L. donovani at
a potent IC 50 of 60 µM.
Abstract
This study describes the synthesis of fluorescent probes as potential substrates for the polyamine transport system (PTS) of Leishmania donovani. A competitive radioassay was used to determine the most efficient probe. We observed that the conjugate spermine-nitrobenzofurazan (Spm-NBD) was able to compete with [3H]-spermidine in L. donovani at a potent IC50 of 60 µM.
Elsevier
